ONTOLOGY SOURCE REFERENCE
Term Source Name	BTO	CHEBI	CHMO	EFO	MI	MTBLS	NCBITaxon	NCIT	OBI	UO
Term Source File	http://purl.obolibrary.org/obo/bto.owl	http://purl.obolibrary.org/obo/chebi.owl	http://purl.obolibrary.org/obo/chmo.owl	http://www.ebi.ac.uk/efo/efo.owl	http://purl.obolibrary.org/obo/mi.owl	https://github.com/EBI-Metabolights/MtblsWS-Py/blob/main/resources/Metabolights.owl	http://purl.obolibrary.org/obo/ncbitaxon.owl	http://purl.obolibrary.org/obo/ncit.owl	http://purl.obolibrary.org/obo/obi.owl	http://purl.obolibrary.org/obo/uo.owl
Term Source Version		249	2025-10-21	3.87.0			2025-12-03	25.06e	2025-12-18	2026-01-16
Term Source Description	The BRENDA Tissue Ontology (BTO)	Chemical Entities of Biological Interest	Chemical Methods Ontology	Experimental Factor Ontology	Molecular Interactions Controlled Vocabulary	MetaboLights Controlled Vocabulary	NCBI organismal classification	NCI Thesaurus OBO Edition	Ontology for Biomedical Investigations	Units of measurement ontology
INVESTIGATION
Investigation Identifier	MTBLS1
Investigation Title	A metabolomic study of urinary changes in type 2 diabetes in human compared to the control group
Investigation Description
Investigation Submission Date	2012-02-14
Investigation Public Release Date	2012-02-14
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INVESTIGATION PUBLICATIONS
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INVESTIGATION CONTACTS
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STUDY
Study Identifier	MTBLS1
Study Title	A metabolomic study of urinary changes in type 2 diabetes in human compared to the control group
Study Description	Type 2 diabetes mellitus is the result of a combination of impaired insulin secretion with reduced insulin sensitivity of target tissues. There are an estimated 150 million affected individuals worldwide, of whom a large proportion remains undiagnosed because of a lack of specific symptoms early in this disorder and inadequate diagnostics. In this study, NMR-based metabolomic analysis in conjunction with uni- and multivariate statistics was applied to examine the urinary metabolic changes in Human type 2 diabetes mellitus patients compared to the control group. The human population were un medicated diabetic patients who have good daily dietary control over their blood glucose concentrations by following the guidelines on diet issued by the American Diabetes Association. Note: This is part of a larger study, please refer to the original paper below.
Study Submission Date	2012-02-14
Study Public Release Date	2012-02-14
Study File Name	s_MTBLS1.txt
Comment[Revision]	3
Comment[Revision Date]	2025-09-02
Comment[Revision Log]	updating data license
Comment[License]	EMBL-EBI Terms of Use
Comment[Study Category]	other
Comment[Template Version]	1.0
Comment[Sample Template]	minimum
STUDY DESIGN DESCRIPTORS
Study Design Type	diabetes mellitus	metabolic syndrome	Urine global profiling	nuclear magnetic resonance spectroscopy	Human Study Subject	untargeted metabolites
Study Design Type Term Accession Number	http://www.ebi.ac.uk/efo/EFO_0000400	http://www.ebi.ac.uk/efo/EFO_0000195		http://purl.obolibrary.org/obo/CHMO_0000591	http://purl.obolibrary.org/obo/NCIT_C70665	http://www.ebi.ac.uk/metabolights/ontology/MTBLS_000279
Study Design Type Term Source REF	EFO	EFO		CHMO	NCIT	MTBLS
STUDY PUBLICATIONS
Study PubMed ID	17190852
Study Publication DOI	10.1152/physiolgenomics.00194.2006
Study Publication Author List	Salek RM,Maguire ML,Bentley E,Rubtsov DV,Hough T,Cheeseman M,Nunez D,Sweatman BC,Haselden JN,Cox RD,Connor SC,Griffin JL
Study Publication Title	A metabolomic comparison of urinary changes in type 2 diabetes in mouse, rat, and human.
Study Publication Status	published
Study Publication Status Term Accession Number	http://www.ebi.ac.uk/efo/EFO_0001796
Study Publication Status Term Source REF	EFO
STUDY FACTORS
Study Factor Name	Gender	Metabolic syndrome
Study Factor Type	Gender	metabolic syndrome
Study Factor Type Term Accession Number	http://purl.obolibrary.org/obo/NCIT_C17357	http://www.ebi.ac.uk/efo/EFO_0000195
Study Factor Type Term Source REF	NCIT	EFO
STUDY ASSAYS
Study Assay File Name	a_MTBLS1_metabolite_profiling_NMR_spectroscopy.txt
Study Assay Measurement Type	metabolite profiling assay
Study Assay Measurement Type Term Accession Number	http://purl.obolibrary.org/obo/OBI_0000366
Study Assay Measurement Type Term Source REF	OBI
Study Assay Technology Type	NMR spectroscopy assay
Study Assay Technology Type Term Accession Number	http://purl.obolibrary.org/obo/OBI_0000623
Study Assay Technology Type Term Source REF	OBI
Study Assay Technology Platform	Nuclear Magnetic Resonance (NMR) - - - Bruker
STUDY PROTOCOLS
Study Protocol Name	Sample collection	Extraction	NMR sample	NMR spectroscopy	NMR assay	Data transformation	Metabolite identification
Study Protocol Type	Sample collection	Extraction	NMR sample	NMR spectroscopy	NMR assay	data transformation	metabolite identification
Study Protocol Type Term Accession Number	http://www.ebi.ac.uk/metabolights/ontology/MTBLS_001100	http://www.ebi.ac.uk/metabolights/ontology/MTBLS_001105	http://www.ebi.ac.uk/metabolights/ontology/MTBLS_001110	http://www.ebi.ac.uk/metabolights/ontology/MTBLS_001111	http://www.ebi.ac.uk/metabolights/ontology/MTBLS_001112	http://purl.obolibrary.org/obo/OBI_0200000	http://purl.obolibrary.org/obo/MI_2131
Study Protocol Type Term Source REF	MTBLS	MTBLS	MTBLS	MTBLS	MTBLS	OBI	MI
Study Protocol Description	For the human studies, midstream urine (~15 ml) samples were collected and frozen from each volunteer. In total, 84 samples were collected from 12 healthy volunteers (7 time points, 8 males and 4 females) and 50 samples from 30 T2DM patients (1-3 time points, 17 males and 13 females) with well-controlled blood glucose maintained at normal concentrations by diet, following the guidelines issued by the American Diabetes Association, rather than medication. T2DM patients agreed to stop treatment with oral anti-diabetic agents during the study. Subjects went through a washout period of 4 wk before sample collection and abstained from alcohol during the study; diet was controlled throughout the study.	For the human studies, midstream urine (∼15 ml) samples were collected and frozen from each volunteer. In total, 84 samples were collected from 12 healthy volunteers (7 time points, 8 males and 4 females) and 50 samples from 30 T2DM patients (1–3 time points, 17 males and 13 females) with well-controlled blood glucose maintained at normal concentrations by diet, following the guidelines issued by the American Diabetes Association, rather than medication. The healthy subjects were aged 18–55 yr, had a body mass index (BMI) ≥19 and ≤30 kg/m2 and a body mass ≥50 kg and ≤113 kg, and were free from any major disease or pregnancy. The T2DM patients were aged 30–65 yr (mean 56 ± 9 yr), had a BMI >25 and <40 kg/m2, weighed between 65 and 140 kg (mean 95 ± 19 kg), and were taking at most one oral anti-diabetic drug. T2DM patients agreed to stop treatment with oral anti-diabetic agents during the study. Subjects went through a washout period of 4 wk before sample collection and abstained from alcohol during the study; diet was controlled throughout the study.	Aliquots of 400 µl urine samples were made up to 600 µl with phosphate buffer (0.2 M, pH 7.4) and any precipitate removed by centrifugation. In total, 500 µl of supernatant were transferred to 5-mm NMR tubes with 100 µl of sodium 3-trimethylsilyl-(2,2,3,3-2H4)-1-propionate (TSP)/D2O/sodium azide solution (0.05% wt/vol TSP in D2O and 1% wt/vol sodium azide).	The spectra of human urine samples were acquired on a Bruker DRX700 NMR spectrometer using a 5 mm TXI ATMA probe at a proton frequency of 700.1 MHz and ambient temperature of 27 °C.	A 1D NOESY presaturation pulse sequence was used to analyze the urine samples. For each sample 128 transients were collected into 64k data points using a spectral width of 14.005 kHz (20 ppm) and an acquisition time of 2.34 s per FID.	Spectra were processed using ACD/1D NMR Manager 8.0 with Intelligent Bucketing Integration (Advanced Chemistry Development, Toronto, ON, Canada). Spectra were integrated 0.20-9.30 ppm excluding water (4.24-5.04 ppm), glucose (3.19-3.99 ppm, 5.21-5.27 ppm), and urea (5.04-6.00 ppm). Intelligent bucketing ensures that bucket edges do not coincide with peak maxima, preventing resonances from being split across separate integral regions; a 0.04 ppm bucket width and a 50% looseness factor were used. All spectra were normalized to total area excluding the water, urea, and glucose regions.	Assignments were confirmed by two dimensional spectroscopy including homonuclear 1H-1H Correlation Spectroscopy (COSY), 1H-13C Heteronuclear Signal Quantum Coherence (HSQC) and 1H-13C Heteronuclear Multiple Bond Correlation (HMBC) Spectroscopy.
Study Protocol URI						http://www.acdlabs.com/products/adh/nmr/1d_man/
Study Protocol Version						ACD nmr manager 8.0
Study Protocol Parameters Name		Extraction Method	NMR tube type;Solvent;Sample pH;Temperature	Instrument;NMR Probe;Number of transients;Pulse sequence name;Magnetic field strength
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STUDY CONTACTS
Study Person Last Name	Salek	Griffin
Study Person First Name	Reza	Jules
Study Person Mid Initials	M	L
Study Person Email	rms72@cam.ac.uk	jlg40@cam.ac.uk
Study Person Phone	01223674948	01223674922
Study Person Fax
Study Person Address	The Department of Biochemistry, The Sanger Building, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.	The Department of Biochemistry, The Sanger Building, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
Study Person Affiliation	University of Cambridge	University of Cambridge
Study Person Roles	principal investigator role	principal investigator role
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